Skip to main content
SpringerLink
Log in

HTB140 melanoma cells under proton irradiation and/or alkylating agents

  • Biophysical Chemistry
  • Published:
Russian Journal of Physical Chemistry A Aims and scope Submit manuscript

Abstract

Chemoresistance is a major problem in the treatment of malignant melanoma. The mainstay of treatment for melanoma is the DNA-alkylating agent dacarbazine (DTIC). Fotemustine (FM), a member of the chloroethylnitrosourea group of alkylating agents, has also demonstrated significant antitumor effects in malignant melanoma. However, the intrinsic and acquired resistance of melanoma limits the clinical application of these drugs. Melanomas are also extremely radioresistant. With the objective of enhancing growth inhibition of melanoma cells, combined treatments of FM or DTIC with proton irradiation have been investigated. These effects were studied on HTB140 melanoma cell viability and proliferation. Cells exposed to treatment with FM and protons have shown inhibition of cell growth and significant reduction of proliferation capacity compared to single irradiation or drug treatment. Treatment with DTIC and protons has shown improved growth inhibition compared to appropriate single drug treatment, while the effects of single proton irradiation have been the most pronounced.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. B. Atkins, Curr. Opin. Oncol. 95(2), 205 (1997).

    Article  Google Scholar 

  2. D. Bafaloucos, D. Tsoutsos, H. Kalofonos, et al., Ann. Oncol. 16, 950 (2005).

    Article  Google Scholar 

  3. R. M. MacKie, Arch. Dermatol. 136, 71 (2000).

    Article  CAS  Google Scholar 

  4. B. J. Sanderson and A. J. Shield, Mutat. Res. 355, 41 (1996).

    Google Scholar 

  5. M. T. Hayes, J. Bartley, P. G. Parsons, et al., Biochemistry 36, 10646 (1997).

  6. M. Christmann, M. Pick, H. Lage, et al., Int. J. Cancer 92, 123 (2001).

    Article  CAS  Google Scholar 

  7. S. M. Lee, D. C. Betticher, and N. Thatcher, Br. Med. Bull. 51, 609 (1995).

    CAS  Google Scholar 

  8. L. Kodijikian, P. Roy, F. Rouberol, et al., Am. J. Ophtalmol. 137, 1022 (2004).

    Google Scholar 

  9. A. Ristić-Fira and I. Petrović, Arch. Biol. Sci. 52(3), 123 (2000).

    Google Scholar 

  10. IAEA Technical Report, Series N 398, 135 (2000).

  11. G. A. P. Girrone, G. Cuttone, P. A. Lojacono, et al., IEEE Trans. Nucl. Sci. 51(3), 860 (2004).

    Article  CAS  Google Scholar 

  12. P. Skehan, R. Storeng, D. Scudiero, et al., J. Natl. Cancer Inst. 82, 1107 (1990).

    Article  CAS  Google Scholar 

  13. K. T. Papazisis, G. D. Geromichalos, K. A. Dimitriadis, and A. H. Kortsaris, J. Immunolog. Methods 208, 151 (1997).

    Article  CAS  Google Scholar 

  14. J. Y. Winum, J. L. Boussiere, I. Passagne, et al., Eur. Med. Chem. 38, 319 (2003).

    Article  CAS  Google Scholar 

  15. J. Rodriguez-Vicente, V. Vicente-Ortega, and M. Canteras-Jordana, Cancer 82, 495–502 (1998).

    Article  CAS  Google Scholar 

  16. M. Sanada, Y. Takagi, R. Ito, and M. Sekiguchi, DNA Repair (Amsterdam) 3, 413 (2004).

    Article  CAS  Google Scholar 

  17. H. Shibuya, Y. Kato, M. Saito, et al., Melanoma Res. 13, 457 (2003).

    Article  CAS  Google Scholar 

  18. I. Petrović, A. Ristić-Fira, D. Todorović, et al., Int. J. Radiat. Biol. 82, 251 (2006).

    Article  CAS  Google Scholar 

  19. I. Petrović, L. Korićanac, D. Todorović, et al., Ann. N. Y. Acad. Sci. 1095, 154–164 (2007).

    Article  CAS  Google Scholar 

  20. J. A. Rafferty, A. R. Clarke, D. Sellappan, et al., Oncogene 12, 693 (1996).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Vinča Institute of Nuclear Sciences, P.O.Box 522, Belgrade, Serbia

    L. Korićanac, I. Petrović & A. Ristić-Fira

  2. Institute of Radiology and Radiation Oncology, University of Catania, Italy

    G. Privitera

  3. LNS, Istituto Nazionale di Fizica Nucleare, Catania, Italy

    G. Cuttone

Authors
  1. L. Korićanac
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Petrović
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Privitera
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Cuttone
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Ristić-Fira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Ristić-Fira.

Additional information

The text was submitted by the authors in English.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Korićanac, L., Petrović, I., Privitera, G. et al. HTB140 melanoma cells under proton irradiation and/or alkylating agents. Russ. J. Phys. Chem. 81, 1467–1470 (2007). https://doi.org/10.1134/S0036024407090233

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0036024407090233

Keywords

Search

Navigation